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iXon EMCCD カメラ

Andorは、科学用に特化したカメラiXon EMCCDの発売を開始しました。iXon EMCCDは、真の意味での高級品で、これまでに例を見ない超高感度科学カメラプラットホームです。主要な性能仕様およびパラメータにEMCCD技術の粋を結集させて特別に設計されました。

新発売iXon Life - 蛍光顕微鏡専用機

  • 単光子感度
  • 背面照射型、QE 95%以上
  • 高フレームレート
  • 特色あるコストパフォーマンス
  • 単分子検出器として究極化
  • 繊細な細胞生理機能を保存
  • Now with SRRF-Stream の超分解能をフル活用
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EMCCD アプリケーション

SRRF解析用SRRF-Stream

SRRFは、新しい リアルタイム超分解能解能顕微鏡検査機能であり、Andor iXon LifeおよびiXon Ultra のEMCCDカメラに搭載されています。

超分解能をすべての人が簡単に利用可能であるべきとのAndorの考えに基づき、既存の蛍光顕微鏡でリアルタイムの超分解能顕微鏡検査を実施するという難問をSRRF-Stream搭載カメラによって解決しました。大部分のデータセットに対し、2から6フォルドへの分解能の向上(最終分解能で50から150nm)が期待されます。

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Single Molecule Detection

For many years, Andor’s iXon EMCCDs have been the gold standard detectors of the biophysics laboratory. To this day they remaining the dominant detector type, operating in a low light regime that is less suited to even back-illuminated sCMOS cameras, especially under the critically demanding conditions of minimized fluorophore photobleaching. The accelerated readout rates of the iXon Life, especially combined with ‘Optically Centred Crop Mode’, means that dynamic single molecule processes can be better characterised.

The 13μm pixel of the 888 model provides superb single molecule resolving capability at the diffraction limit, while preserving optical photon collection efficiency.

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Physiology / Ion Imaging & Cell Motility

Unsurpassed EMCCD sensitivity and superb custom ROI speeds make the iXon Life 888 and 897 the best possible detectors for temporally resolving fast calcium sparks and waves under minimal dye concentrations, critical to reducing the ‘dye buffering effect’ that can distort the very physiology that is under study.

Light levels become more challenging still when combined with low light optical sectioning modalities, making EMCCD ultrasensitivity all the more necessary. The same EMCCD advantages play equally well to imaging of the motile cell.

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Spinning Disk Confocal Microscopy

The iXon Life 888 is the ideal detector to drive superlative performance from confocal spinning disk technology. Whilst affording superb confocality and low rates of phototoxicity, spinning disk experiments are inherently photon starved, by virtue of the photon rejection implicit to optical sectioning.

The superior sensitivity of the iXon Life detector brings these low light images to life.

Note, often high-end confocal systems are equipped with a second Andor Zyla sCMOS camera to access the highest possible resolution and field of view, provided the sample is sufficiently bright.

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Quantum Physics & BEC

Quantum entanglement occurs when two particles remain connected, even over large distances, so that actions performed on one particle have an effect on the other. Quantum entanglement understanding is the basis of the growing fields of quantum computing and quantum cryptography.

The iXon Ultra EMCCD platform is a well established platform for single photon detection. With deep cooling down to -100 °C and minimized Clock Induced Charge (CIC) noise, the Ultra provides the single photon counting discrimination required for successful detection in these demanding experiments.

The iXon Ultra has for many years been the detector of choice in cold atom/ion trap experiments. With EMCCD and CCD amplifiers, the camera can be readily adapted to both fluorescence and absorption BEC set-ups. Further more, the Ultra can be optimized for enhanced NIR detection and/or fringe suppression, along with a custom AR-coated entrance window.

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Fast Astronomy

Featuring single photon sensitivity, TE cooling to -100 °C and > 95% QE, the iXon Ultra EMCCD range has been central to many demanding observing initiatives, such as the RAVEN ‘Multi-Object Adaptive Optics’ system at the Subaru Telescope (Hawaii), CHIMERA, the Caltech high speed multi-color camera for detecting transiting objects in the Kuiper Belt and as a guide camera and science camera on SOFIA, the Stratospheric Observatory for Infrared Astronomy (pictured).

The dual EMCCD and CCD capability of the larger format iXon Ultra 888  model extends the application usage of this versatile camera.

Key iXon EMCCD applications within astronomy include AO wavefront sensing, high time resolution astrophysics, speckle imaging and transit surveys.

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Bioluminescence Microscopy

The advantage of bioluminescence microscopy over fluorescence microscopy relies on the absence of autofluorescent background, and, since no light source is necessary to excite the probe, photobleaching or phototoxic effects are remarkably reduced. However, bioluminescent proteins have relatively weak brightness compared with fluorophores. Ideal detectors should thus have high quantum efficiency and reduced source of noise in order to optimize the detection limit.

iXon Ultra offers the best-in-class solution for ultrasensitive bioluminescence imaging across a range of exposure conditions. Single photon sensitivity, back-illuminated QE and deep TE cooling combine to shorten exposure times, such that high quality faster time-lapse imaging is easily achievable. ‘2-in-1’ choice of EMCCD and CCD modes further enhance application flexibility.

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iXon UltraモデルとiXon Lifeモデルの違い

iXon Ultra 

EMCCD技術の粋を集積

市場をリードするEMCCDカメラであるAndor iXon Ultraは、微弱光という厳しい条件のアプリケーションに対して優れた性能を発揮する万能型ソリューションとして評価されています。iXon Ultraは、市場最高レベルのTE冷却温度、EMCCDおよびCCDの同時読出しモードなどの優れた機能を備え、物理科学および生命科学分野のアプリケーション対応機として他に類を見ない柔軟性と機能性を提供します。

New iXon Life

低価格で高性能を実現

新発売のAndor iXon Life EMCCDモデルは、蛍光顕微鏡検査用に特化したモデルであり、コストパフォーマンスを見事に改善した単光子検出感度が備わるよう設計しました。光毒症および光退色を最小化させるという点で単分子および生細胞の顕微鏡検査用としての理想形を追求する一方で、これまでの高性能EMCCDカメラの概念を覆す価格を実現しました。

iXon Ultra  iXon Life 
従来型CCDアンプ Yes -
組込み型シャッタ Yes  -
Min. TE冷却温度 -100 °C -80 °C
直接データアクセス(CameraLink)  Yes
EMCCDモード読出し速度 888 model - 30, 20, 10 & 1897 model - 17, 10, 5 & 1 888 model - 30 & 10897 model - 17 & 10
単光子計数モード Yes -

iXon モデルのオプション

表に示す各モデルは、UltraおよびLifeのいずれのプラットホームにおいても利用できます。

最先端の技術とその利点

電子増倍CCD(EMCCD)は、いかなる読出し速度であっても読出しノイズレベルを抑えながら微弱な信号事象をある信号レベルまで増倍すること(EMゲイン)により作動するため、これにより単光子の感知が可能となります。

高QE‐低ノイズを追求する最近の技術革新の流れの中で、低ノイズsCMOS技術が広範囲な科学用途に採用されています。ただし超微弱光という条件において必要な本来の感度はEMCCDのみが提供可能であり、超微弱光の克服を課題とするアプリケーションにとっては重要な利点となります。

Andor’s market leading iXon design ensures the absolute highest sensitivity from a quantitative scientific digital camera, particularly under fast frame rate conditions.

Andor’s proven UltraVac™ vacuum technology, carrying a 7 year warranty, is critical to ensure both deep cooling and complete protection of the sensor. The iXon series is designed to be the most flexible yet easy to use EMCCD camera on the market, optimizable for a wide variety of application requirements in a single click via the OptAcquire™ feature. Furthermore, signal can be quantitatively calibrated in units of electrons or photons, either real time or post-processing.

 

Single Molecule Detection – single molecule experiments present us with the considerable challenge of harnessing the limited photon budget from dynamic, individual fluorescent molecules, while also avoiding photobleaching. Andor iXon EMCCDs have continued to radically out-perform sCMOS detectors in this most demanding of light starved application areas.

Super-Resolution – SRRF-Stream is a NEW real time super-resolution microscopy functionality that operates on Andor’s iXon EMCCD cameras. ‘SRRF-Stream unlocks the means to perform real time super resolution microscopy on conventional modern fluorescence microscopes. Resolution improvement from 2- to 6-fold (50-150nm final resolution) can be expected for most datasets.

Reduced phototoxicity – iXon facilitates use of lowest possible excitation power while maintaining superb signal to noise ratio, minimizing phototoxic effects. No other camera is this good at preserving your cell’s physiological behaviour over extended periods of measurement.

Lowest fluorophore concentrations – There is an ongoing drive in fluorescence microscopy to push to lower and lower fluorophore concentrations in order not to perturb the physiology of the living cells being studied. iXon’s superior sensitivity facilitates use of unprecedentedly low label concentrations, thus minimizing the ‘observer effect’.

Single Photon Counting – iXon Ultra presents the absolute lowest noise levels possible, providing the level of discrimination needed to confidently detect and register single photon events, ideal for demanding quantum physics applications such as entangled photon studies.

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